Agro-Food and Lignocellulosic UrbanWastes as Sugar-Rich Substrates for Multi-Product Oil-Based Biorefineries

Abstract

The effective use of biowaste resources becomes crucial for the development of bioprocessing alternatives to current oil- and chemical-based value chains. Targeting the development of multi-product biorefinery approaches benefits the viability and profitability of these process schemes. Certain oleaginous microorganisms, such as oleaginous red yeast, can co-produce industrially relevant bio-based products. This work aims to explore the use of industrial and urban waste as cost-effective feedstock for producing microbial oil and carotenoids using Rhodosporidium toruloides. The soluble fraction, resulting after homogenization, crushing, and centrifugation of discarded vegetable waste, was used as substrate under a pulse-feeding strategy with a concentrated enzymatic hydrolysate from municipal forestry residue obtained after steam explosion pretreatment (190 ºC, 10 minutes and 40 mg H2SO4/g residue). Additionally, the initial nutrient content was investigated to enhance process productivity values. The promising results of these cultivation strategies yield a final cell concentration of 36.4-55.5 g/L dry cell weight (DCW), with an intracellular lipid content of up 42-45% (w/w) and 665-736 µg/g DCW of carotenoids. These results demonstrate the potential for optimizing the use of waste resources to provide effective alternative uses to current biowaste management practices, also contributing to the market of industrially relevant products with lower environmental impacts.