Evaluation and identification of key economic bottlenecks for cost-effective microbial oil production from fruit and vegetable residues

Abstract

Intensive horticultural systems for the production of vegetables in greenhouses represent one of the main industries generating organic waste, as those that do not meet the quality standards for the fresh market or the processing industry are discarded. This highlights the importance of using these residues as raw material for other applications, such as bioenergy and bioproducts production, within the framework of a bio-based economy that maximizes the utilization of biomass resources in a sustainable manner. In this work, the microbial oil production from discarded pepper using the oleaginous yeast Cryptococcus curvatus was evaluated. Overall, a total lipid accumulation of 16.8 g/L was achieved with a fatty acid profile suitable to produce biodiesel. The lipid yield obtained was 0.12 g/g sugars. In addition, experimental results were used to assess the techno-economic feasibility of a proposed microbial oil plant using the software Aspen Plus. This plant yields approximately 96 kg of microbial oils/ton dry discarded pepper, with an estimated Minimum Selling Price of 7 €·kg−1. These figures point out the necessity of increasing the yield of microbial oil production and considering the utilization of possible by-products, such as mannitol and cell debris, to improve the economic performance of the process.