Higher proliferative capacity of T lymphocytes from patients with Crohn disease than from ulcerative colitis is disclosed by use of Herpesvirus saimiri-transformed T-cell lines

Abstract

lymphocytes play a crucial role in the pathogenesis of inflammatory bowel disease.

Achieving stable T-cell lines, rather than continuous bleeding of patients, is desirable in order to dissect Achieving stable T-cell lines, rather than continuous bleeding of patients is desirable in order to dissect

their implication in the disease. Methods: Long-lasting T-cell lines from patients with Crohn disease and

ulcerative colitis and from healthy volunteers have been obtained by transformation of T lymphocytes

using the lymphotropic nodstts

Herpesvirus saimiri. Lines were subjected to phenotypic and functional analyses, were

and the results compared with freshly isolated peripheral blood mononuclear cells. Results: Fresh cells

revealed only minor differences between patients and controls, with regard to phenotype and proliferative revealed only minor differences between patients and controls, with reeard to phenotyne and proliferative

capacity. In contrast, the use of T-cell lines ls showed that cellss from Crohn disease patients, but not

ulcerative colitis patients, over-responded to several membrane or cytoplasmic stimuli when compared to

control T-cell lines. Thus, higher responses were found when stimulated with αCD3 and IL2, αCD3 and

CD28 12 alone horhol estere (PMA) nd CD3 and fnally PMA and 2005 11 αCD28, IL2 alone, phorbol esters (PMA) and αCD3 and, finally, PMA and αCD2 (P< 0.05 in all

instances). Further, lines from patients with Crohn disease responded more vigorously to αCD3 and

αCD28 or αCD3 and PMA when compared to ulcerative colitis (P<0.05 in both instances). Conclusiosded wimhese thatcels sions: The data obtained with these lines suggest that T cells from patients with Crohn disease differ in

vivo in their proliferative capacity, as compared with those from ulcerative colitis patients, a finding that

may reflect the clear Th-1 phenotyne found in the former and absent in the latte