Environmental risk assessment of neonicotinoids in surface water

Abstract

Neonicotinoids (NNIs) are active substances used as insecticides mainly in plant protection products (PPPs) but also in veterinary applications. The increasing evidence of affecting non-targeted organisms led the European Commission to severely restrict or even ban outdoor uses. To evaluate their current use and their influence in the ecological status of freshwater ecosystem, a total of 19 river water samples were collected to determine the presence of 5 NNIs (acetamiprid, clothianidin, imidacloprid, thiamethoxam and thiacloprid) in the Tagus basin. At least one target analyte was quantified by HPLC-MS/MS analysis in 17 of the 19 water samples, with ΣNNIs ranging from <MDL to 16.8 ng/L. Imidacloprid (2.75 ng/L; mean) and acetamiprid (0.47 ng/L) were quantified in most of the samples. Source identification evidences imidacloprid agricultural use. Risk assessment for different trophic levels was conducted with the data obtained calculating Risk Characterization Ratios (RCR) by two approaches, predicted non effect concentrations (PEC/PNEC) and Toxic Units (TU). RCRs were derived for each NNI and for the mixture of all (RCRmix). Results showed risk for imidacloprid in freshwater organism (RCRfw>1) and for the mix of NNIs (RCRmix (PEC/PNEC) > 1). RCRmix(PEC/PNEC) and the sum of toxic units (STU) showed a risky situation for some locations with different organisms related to agriculture practices. This data arouses concern about NNis (legal or forbidden) use in Tagus basin, and manifest the need of monitoring their presence and effect on the aquatic ecosystem.